Isolation and Screening of Protease Producing Bacteria from Soil

Proteases execute a large variety of functions and have important biotechnological applications. Proteases represent one of the three largest groups of industrial enzymes and find application in detergents, leather industry, food industry, pharmaceutical industry and bioremediation processes. In the present study soil sample is collected from college primesis and used for screening of protease producing bacteria. On the basis of colony size, texture, and microscopic characteristics, the isolates were identified as Bacillus sps .The two Isolated Bacillus sps were screened for protease producing ability on casein agar and gelatine agar plates. The zone formation around the bacterial colony indicated the protease positive strain which may be due to hydrolysis of casein and gelatine. Hence the strains were identified as a protease producer and it was taken for further experimental studies and biochemical test. The extraction of protease enzyme is important due to its wide industrial applications.

Keywords: Proteases, Bacillus sps, Enzyme, Proteolytic Ubiquitous