Isolation and Identification of Nah-B Gene from Bacteria Isolated from Effluents & Testing its Hydrocarbon Degrading Efficiency

One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxin organic pollutants. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. In this project hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil by using nutrient agar medium and mineral salt agar medium supplemented with liquid petrol and diesel which was used as the sole carbon source for bacterial growth was performed. The bacteria was identified by using Bergey’s manual of bacterial classification. The nah gene which responsible for the hydrocarbon degradation was amplified. The sequence has similarity about 90% to nahB gene