Micropropagation of Terminalia Arjuna Roxb., from Nursery Plant Material

ABSTRACT

An in vitro micropropagation system has been developed for Terminalia arjuna Roxb., an important Indian medicinal plant and non mulberry primary food plant of tropical tasar silkworm (Antheria mylitta Drury). Nodal segments obtained from 15-d-old aseptically grown seedlings were used as explants. MS medium containing 1.5 mg/L BAP was found most suitable for culture initiation. Although shoot multiplication was achieved on MS medium containing BAP and Kn, the maximum number of shoots was obtained with 1.5 mg/L BAP. Best rooting response was observed on MS medium salts, 0.6% agar and 0.1 mg/L IBA. Plantlets were hardened initially in culture room conditions and then transferred to misthouse.

Keywords: Axillarys bud proliferation, Arjun, in vitro, rooting, shoot multiplication