Prevalence and Detection of Amp C β- Lactamase Resistance in Gram Negative Uropathogens
International Journal of Emerging Trends in Science and Technology,
Vol. 2 No. 06 (2015),
31 May 2015
Abstract
The most common mechanism of resistance in Gram negative bacteria is by the production of ß lactamases which inactivate ß lactam antibiotics. Among which Amp C β lactamases are more important because they confer resistance to Cephalosporins, β lactam β lactamase inhibitor combinations and Aztreonam. Although reported with increasing frequency, the true rate of occurrence of AmpC β-lactamases remains unknown. The present study was designed to determine the occurrence of AmpC enzyme-harboring Gram-negative uropathogens in a tertiary care hospital. A total of 430 consecutive, non repeat urinary Gram negative isolates were included in the present study. All the 430 isolates were screened for AmpC β-lactamase production by Novel predictor disk placement method. Isolates positive in the screening were further confirmed by inhibitor based Cefoxitin –Cloxacillin disk diffusion test. Sixty eight (16%) isolates were positive for AmpC β-lactamases. Based on the species 36 (14%) Esch.coli ,27 (28%) Klebsiella pneumoniae, 2 (6%) Pseudomonas aeruginosa 1 (5%) Citrobacter freundii, 1 (8%)Acinetobater sp and 1 (33%)Enterobacter aerogens harbored AmpC enzymes. All the isolates were sensitive to imepenam. This study has shown the occurrence of Amp C β lactamase producing Gram negative urinary isolates in our hospital. Failure to identify Amp C β lactamase producers may lead to inappropriate antimicrobial treatment . Inhibitor based Cefoxitin -Cloxacillin disk diffusion test is easier to perform routinely to detect Amp C β lactamase production.
Key words: Gram negative uropathogens, drug resistance, Amp C β-lactamasesHow to Cite
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References
2. Bobak Pourakbari,Farzad Ferdosian,Shima Mahmoudi. Increase resistance rates and ESBL production between E.coli isolates causing urinary tract infection in young patient from Iran.Brazilian Journal of microbiology (2012) 766-769.
3. A.K. Ratna, I. Menon, I. Kapur & R. Kulkarni Occurrence & detection of AmpC β lactamases at a referral hospital in Karnat-aka Indian Med Res 118,July 2003,pp 29-32
4. Bush, K., G. Jacoby, and A. Medeiros, A functional classification scheme for beta-lactamases and its correlation with molec-ular structure. Antimicrobial Agents and Chemotherapy, 1995. 39(6): p. 1211-1233.
5. Varsha Gupta An update on newer β lactamases Indian J Med Res 126, November 2007, pp 417-427
6. Alain Philippon, Guillaume Arlet, and George A. Jacoby Plasmid-determined AmpC Type βLactamases Antimicrobial agent and chemotherapy Jan 2002 Vol. 46, No. 1.
7. Yi Li, Qing Li,Yuzhen Du, Xiaofei Jiang, Jin Tang, Jianqiang Prevalence of Plasmid-Mediated AmpC β-Lactamases in a Chinese University Hospital 2003 to 2005: First Report of CMY-2-Type AmpC β-Lactamase Resistance in China.Journal of Clinic Microbiology, Apr 2008, p. 1317-1321, Vol. 46, No. 4
8. S. Peter-Getzlaff, S. Polsfuss, M. Poledica Detection of AmpC Beta-Lactamase in Escherichia coli: Comparison of Three Phenotypic Confirmation Assays and Genetic Analysis. Clin Microbiol. Aug 2011; 49(8): 2924–2932
9. Mitesh H. Patel, Grishma R. Trivedi, Sachin M. Patel, and Mahendra.Antibiotic susceptibility pattern in urinary isolates of gram negative bacilli with special reference to AmpC β-lactamase in a tertiary care hospital
10. C.Rodrigues,P.Joshi, S.H.Jani, Detection of beta lactamases in nosocomial gram negative clinical isolates,Indian journal of medical microbiology 2004 22 247-250
11. Neena V Nagdeo1, Navinchandra M Kaore2, Vilas R Thombare.Phenotypic methods for detection of various β-lactamases in Gram-negative clinical isolates: Need of the hour Journal of Clinical Microbiology Year : 2012 | Volume : 3 | Issue : 4 | Page : 292-298
12. V.Hemalatha, M. Padma,Uma sekar. Detection of Amp C beta lactamases production in Escherichia coli & Klebsiella by an inhibitor based method.Indian journal of medical research 126, September 2007, p 220-223
13. Mai M. Helmy, and Reham Wasfi. Phenotypic and Molecular Characterization of Plasmid Mediated AmpC β-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospital. BioMed Research International Volume 2014 (2014), Article ID 171548, 8 pages
14. A. Subha, V. Renuka Devi & S. Ananthan. AmpC β lactamase producing multidrug resistant strains of Klebsiella spp. & Escherichia coli isolated from children under five in Chennai Indian J Med Res 117, January 2003, pp 13-18
15. Vikash Manchanda,Narendra P.Singh, Occurrence and detection of Amp C β lactamases among Gram negative clinical isolates using a modified three dimensional test at Guru Tegh Bahadur Hospital, Delhi, India.
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